Transdermal Transport of a Different Size FTIC-dextran using Laser Energy - Results of in Vitro Experiments

Many advantages of transdermal drug delivery have led to investigation into a range of methods that are capable of overcoming the considerable barrier properties of the skin. Different physical enhancement methods are used for this purpose, one of them being the use of fractional lasers to create transport pathways in the stratum corneum and enhance transdermal delivery of drugs [1, 2]. he aim of the study was to demonstrate in vitro that different types and energies of laser pulses greatly influence transdermal transport of FITC-dextran through pigs’ ear skin. Vertical glass Franz diffusion cells were used to study molecular transport through excised and dermatomed pigs’ ear skin. The donor compartment contained 10 kDa FITC-dextran solution (0.1 mM) in phosphate buffer at pH 7.4. The receiver solution was a phosphate buffer (pH 7.4) thermo-regulated at 37°C. The concentration of FITC-dextran in the receiver compartment after laser treatment was measured with a spectrofluorometer.